ICS 65.020 B 62 备案号： 湖 DB42 北 省 地 方 标 准 DB42/T 1448—2018 蝴蝶兰组培苗工厂化生产技术规程 Code of practice for tissue culture of phalaenopsis seedling in industrial production （报批稿） 2018-09-11 发布 湖北省质量技术监督局 2018-12-10 实施 发 布 1 DB42/T 1448—2018 目 次 前言 ................................................................................ III 1 范围 ................................................................................................................................................................ 1 2 规范性引用文件 ............................................................................................................................................ 1 3 术语与定义 .................................................................................................................................................... 1 3.1 组培苗 in vitro plantlet ........................................................................................................................ 1 3.2 中苗 medium sized plantlet .................................................................................................................. 1 3.3 成品苗 the finished product plantlet .................................................................................................... 1 4 设施条件 ........................................................................................................................................................ 1 5 培养基配制 .................................................................................................................................................... 2 5.1 水质要求 ................................................................................................................................................. 2 5.2 培养基配制及分装 ................................................................................................................................. 2 5.3 培养基灭菌 ............................................................................................................................................. 2 5.4 培养基储存 ............................................................................................................................................. 2 6 组织培养操作方法 ........................................................................................................................................ 2 6.1 操作前准备 ............................................................................................................................................. 2 6.2 外植体选择与消毒 ................................................................................................................................. 2 6.3 增殖与继代培养 ..................................................................................................................................... 3 6.4 壮苗培养 ................................................................................................................................................. 3 6.5 生根培养 ................................................................................................................................................. 3 6.6 接种 ......................................................................................................................................................... 4 7 组培苗培养管理 ............................................................................................................................................ 4 7.1 组培苗摆放 ............................................................................................................................................. 4 7.2 培养条件 ................................................................................................................................................. 5 8 炼苗与移栽 .................................................................................................................................................... 5 9 报损预防 ........................................................................................................................................................ 5 9.1 预防原则 ................................................................................................................................................. 5 9.2 降低污染 ................................................................................................................................................. 6 10 包装、标识和储运 ................................................................................................................................